lec1_5 - How exactly does DNA synthesis work? Coupling...

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Unformatted text preview: How exactly does DNA synthesis work? Coupling efFciency? How do you build genes? 1 Cite as: Drew Endy. Course materials for 20.109 Laboratory Fundamentals in Biological Engineering, Fall 2007. MIT OpenCourseWare (http://ocw.mit.edu), Massachusetts Institute of Technology. Downloaded on [DD Month YYYY]. Image removed due to copyright restrictions. Excerpts from: Compton, K. T., and J. W. M. Bunker (MIT). "The Genesis of a Curriculum in Biological Engineering." The Scientific Monthly (January 1939). Cite as: Drew Endy. Course materials for 20.109 Laboratory Fundamentals in Biological Engineering, Fall 2007. MIT OpenCourseWare (http://ocw.mit.edu), Massachusetts Institute of Technology. Downloaded on [DD Month YYYY]. Cite as: Drew Endy. Course materials for 20.109 Laboratory Fundamentals in Biological Engineering, Fall 2007. MIT OpenCourseWare (http://ocw.mit.edu), Massachusetts Institute of Technology. Downloaded on [DD Month YYYY]. To build section alpha, we frst cloned parts 5, 6, 7, 8, 12, 13, 14, 15, 16, 18, 20, 22, and 24 into pSB104. We cloned part 11 into pSB2K3. We cloned each part with its part-specifc bracketing restriction sites surrounded by additional BioBrick restriction sites. We used site-directed mutagenesis on parts 6, 7, 14, and 20 to introduce the sites U1, U2, U3, and U4, respectively. Our site-directed mutagenesis oF part 20 Failed.We used site-directed mutagenesis to remove a single Eco0109I restriction site From the vector Failed....
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This note was uploaded on 11/11/2011 for the course BIO 20.010j taught by Professor Lindagriffith during the Spring '06 term at MIT.

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lec1_5 - How exactly does DNA synthesis work? Coupling...

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