lec2_5 - Module 2: Expression Engineering 20.109 Lecture 5...

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Unformatted text preview: Module 2: Expression Engineering 20.109 Lecture 5 October 25th, 2007 Cite as: Natalie Kuldell. Course materials for 20.109 Laboratory Fundamentals in Biological Engineering, Fall 2007. MIT OpenCourseWare (http://ocw.mit.edu), Massachusetts Institute of Technology. Downloaded on [DD Month YYYY]. Expression Engineering Experiment Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Image of glowing luciferase in a microcentrifuge tube, removed due to copyright restrictions. RT Figure by MIT OpenCourseWare. Image credits: Day 1 - Courtesy of Arkitek Studios. Used with permission. Day 2 - Courtesy of The Exploratorium. The Exploratorium, http://www.exploratorium.edu. Day 4 Figure by MIT OpenCourseWare. Day 5 Dr. Natalie Kuldell. Day 6 Courtesy of NIH. Cite as: Natalie Kuldell. Course materials for 20.109 Laboratory Fundamentals in Biological Engineering, Fall 2007. MIT OpenCourseWare (http://ocw.mit.edu), Massachusetts Institute of Technology. Downloaded on [DD Month YYYY]. Expression Engineering Experiment Lecture 1 Lecture 2 intro to cell culture transfection intro to gene expn/RNAi luciferase Lecture 3 Lecture 4 off-target/nonspecific RNAi Writing lecture (Neal Lerner) Lecture 5 Lecture 6 measuring gene expressn microarray analysis (Rebecca Fry) Lecture 7 Lecture 8 high throughput technologies RNAi applications (no lab) or review of your data Cite as: Natalie Kuldell. Course materials for 20.109 Laboratory Fundamentals in Biological Engineering, Fall 2007. MIT OpenCourseWare (http://ocw.mit.edu), Massachusetts Institute of Technology. Downloaded on [DD Month YYYY]. DNA/RNA/Protein Compass N S E W Cite as: Natalie Kuldell. Course materials for 20.109 Laboratory Fundamentals in Biological Engineering, Fall 2007. MIT OpenCourseWare (http://ocw.mit.edu), Massachusetts Institute of Technology. Downloaded on [DD Month YYYY]. DNA/RNA/ Protein Compass 25 M13 Candidate 2 (colony #4) 25 M13 Candidate 1 (colony #3) 25 M13k07 (positive control) 5 Marker Volume loaded (ul) Sample 25 M13 Candidate 2 (colony #4) 25 M13 Candidate 1 (colony #3) 25 M13k07 (positive control) 5 Marker Volume loaded (ul) Sample Figure 2 Western Blot 50 KD 37 KD P3 (~48KD) Figure 2 Protein samples from bacteria infected with modified an d unmodified M13 virus ran in a polyacrylamide gel. Antibodies with alkaline phosphatase were used to identify virally encoded protein p3. W e see strong bands at the expected length of p3 for our control and experimental samples which suggests our modified viruses successfully induced p3 production in their hosts. AB s used: Primary: mouse anti -P3, Secondary: Goat anit-mouse with alkaline phosphatase . from Augusto Tentori N S E W Courtesy of Augusto Tentori. Used with permission....
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lec2_5 - Module 2: Expression Engineering 20.109 Lecture 5...

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