lecture12

lecture12 - 20.106J Systems Microbiology Lecture 12 Prof....

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20.106J – Systems Microbiology Lecture 12 Prof. DeLong ± Chapter 15 – Brock ± Demonstration: Prof. Delong shows us a 48 capillary array DNA sequencing device with an optical detector. o You can do some 500 runs on the same polymer o Then Prof. Delong shows us a 1.5 million well plate – you can do 1.5 million sequencing reactions all at once ± Each well is around 60 microns across, and you get one bead in each well ± Genomics II o Comparative genomics basics o Gene calling o Bioinformatics web resources – there are a huge number of tools o Genomics of insect endosymbionts ± We each have around 10 14 microbes associated with us, that we carry around in our bodies o We all have our own genes that predispose us to certain diseases o It’s not that well understood how the human-associated microbe interacts with human genetics o There are some really neat studies going on right now involving how mice genetics determine which microbes live in the mouse’s gut ± Genomics is the starting point ± What do we do with a sequence, once assembled? o The first thing to do is to translate it into the reading frame – look at which codons, and start to discover what the genes are o The range of the number of base pairs and genes stretch from around 400 protein encoding genes up to around 10,000. ± The organisms with the fewest genes are obligate endosymbionts
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This note was uploaded on 11/11/2011 for the course BIO 20.010j taught by Professor Lindagriffith during the Spring '06 term at MIT.

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lecture12 - 20.106J Systems Microbiology Lecture 12 Prof....

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