lecture15

lecture15 - 20.106J Systems Microbiology Lecture 15 Prof....

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20.106J – Systems Microbiology Lecture 15 Prof. DeLong ¾ Exam this week: ¾ Lectures Oct 23 (techniques of genomics), 25 (comparitive genomics), 29 (lateral gene transfer and how genomes evolve), and Nov 1 (Nitrogen cycle from a global perspective, rhizobium and agrobacterium) ¾ Brock Chapters 15 (all), 17(586-591), 19 (656-666), and 31 (989-991) ¾ Today’s lecture won’t be on the exam. ± Environmental Genomics and Microbial Ecology o Natural microbial diversity o Molecular microbial ecology techniques o Environmental Genomics ± Methods for trying to understand bacterial communities – we don’t normally get pure cultures o If you get a pure culture, it’s much easier to describe o But then you have to be able to describe how it interacts with the larger mixed environment that it came from o Counting: ± Seawater plate count (hundreds of cells per ml) ± Direct count – fluorescence (millions of cells per ml) ± Why do we get this difference? ± If there are all these microbes that won’t grow on a plate culture in the lab, then how can we characterize and study them? o
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lecture15 - 20.106J Systems Microbiology Lecture 15 Prof....

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