MIT20_020S09_prj8_hepc_sp

MIT20_020S09_prj8_hepc_sp - By Jenny Drawing of Hepatitis C...

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Unformatted text preview: By Jenny Drawing of Hepatitis C virus structure Leanna removed due to copyright restrictions. Samira Tech Spec Review Hepatitis C Treatment + Introduction What is hepatitis C? Why we chose it? + How it works? + Chimp study- why nucleosides Study tested in vivo effects of nucleoside in chimpanzees Injecting 0.2-2 mg of nucleoside/ kg of body weight for 12 days Found that there was a >5log10(original IU concentration) decrease to the point where they could no longer detect the presence of IU Improvement through cell uptake Image courtesy of Ori2uru on Flickr. + Model Structure of HCV + System level diagram + Figures by MIT OpenCourseWare. + Engineering the capsid producing DNA + + Engineering nucleoside RNA - 1 + Engineering nucleoside RNA - 2 RNA for nucleoside production + Time Diagram Within liver cell culture Collection and Injection of debuggers + Parts list In Vitro P arts Sourcing Description Annotation Liver Cells PromoCell Human Hepatocyte Will produce HCV capsids DNA (Capsid, E1, E2, GFP, terminator) Integrated DNA Technologies Deoxyribonucleic acid Codes for capsid and proteins Tet repressor sequence from Reg. Standard Biological Parts Controls expression of our DNA When Tet is present, gene will not be expressed Doxycycline Next Tag Antibiotic Allows DNA to be expressed Capsid, E1, E2 Sequence from National Center for...
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MIT20_020S09_prj8_hepc_sp - By Jenny Drawing of Hepatitis C...

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