MIT20_020S09_prj23_trsh_sp

MIT20_020S09_prj23_trsh_sp - Trash to Treasure:...

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Trash to Treasure: Accelerating Composting Anonymous students CL and SJ
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Compost: The Problem Naturally takes a year Bacteria operate within different temperature zones 0-40°C – mesophilic topsoil bacteria 60 40-55 °C – thermophilic bacteria ~ similar to hot- 50 40 springs 30 20 Actinomycetes 10 Dirt smell 0 Breaks down complex organics Temp ( O C) Compost temperature Time Figure by MIT OpenCourseWare.
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Goal: Accelerate the speed of composting
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Solution: Compost Pearls
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Description Pearl covered with 2 cell types: Type A and Type B Type A has cellulase over-expression Lyses and releases cellulase into surrounding area Type B converts cellulose to hydrocarbons Uses pathway of Gliocladium roseum Spongy Core to trap hydrocarbons when they are produced Metal at the center for easy retrieval
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Overall System
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2 Methods E-coli Uses predator-prey model to regulate level of strain A compared to strain B Yeast Uses mother-daughter cells to regulate level of strain A compared to strain B Courtesy of Sajith Wickramasekara. Used with permission.
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MIT20_020S09_prj23_trsh_sp - Trash to Treasure:...

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