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MIT20_020S09_proj14_nrg_sp

MIT20_020S09_proj14_nrg_sp - Team ENERGYneering Te Growing...

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Team ENERGYneering Team ENERGYneering Team Members: Ragheb El Khaja, Abi Jain, and anonymous students SC and TM Growing Green
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Goal: How can we help solve the Goal: How can we help solve the world world s energy problems?? s energy problems?? Find a way to maximize the production of hydrocarbons Find a way to maximize the harvest (yield) of hydrocarbons
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Novelty of Our System Novelty of Our System… Why Algae Why Algae E coli Must be forced to produce fuels Algae Optimized for the production of fuel Yeast Produces ethanol (inferior) Problem: export is extremely difficult
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Novelty of Our System Novelty of Our System… Possible Solutions Possible Solutions How do we harvest the hydrocarbons? Option 1: Lyse the cells (possibly with a phage gene) Option 2: Continuous export (through metabolic engineering)
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Novelty of Our System Novelty of Our System… Metabolic engineering Metabolic engineering Goal: force hydrocarbons to be secreted from the algae How? Unblock the path by minimizing cell wall Increase the production of hydrocarbons Ramp up hydrocarbon pathway Cut off carbon diversion to other pathways
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Which species of algae? Which species of algae? C. reinhardtii Use as a model for our system Is already sequenced Successful homologous recombination B. braunii Our ideal species Has most productive fuel pathways Produces hydrocarbons vs fatty acids Problem : has not been sequenced Photo removed due to copyright restrictions.
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