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orientation - Welcome to 20.109 Laboratory Fundamentals of...

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Unformatted text preview: Welcome to 20.109 Laboratory Fundamentals of Biological Engineering Orientation Lecture Fall 2007 20.109 Laboratory Fundamentals of Biological Engineering Course Mission To prepare students to be the future of Biological Engineering To teach cutting edge research skill and technology through an authentic research experience To inspire rigorous data analysis and its thoughtful communication Module 1 Module 2 Genome Engineering Expression Engineering Module 3 Biomaterials Engineering Cite as: Angela Belcher, Drew Endy, Natalie Kuldell and Agi Stachowiak. Course materials for 20.109 Laboratory Fundamentals in Biological Engineering, Fall 2007. MIT OpenCourseWare (http://ocw.mit.edu), 1 Massachusetts Institute of Technology. Downloaded on [DD Month YYYY]. Genome Engineering: M13 redesign Courtesy of Elsevier, Inc. http://www.sciencedirect.com. Used with permssion. Source: Specthrie, L., et al. “Construction of a Microphage Variant of Filamentous Bacteriophage.” J Mol Biol 228, no. 3 (December 5, 1992): 720-4. Lab skills Experiments Modify existing genome to tag one coat protein Refactor genome, order its synthesis and test Compare infection of minimal and robust E . coli genome DNA manipulations • digests • transformation • electrophoresis Western analysis Cell & phage culture Expression Engineering: siRNA Left: Firefly photo courtesy of Eloise Mason. Used with permission. Center: Plasmid map courtesy of Promega Corporation. Used with permission. Right: “Sea pansy” (Renilla reniformis) courtesy of NOAA. Used with permission. Experiments Silence expression of a gene using Lab skills Genetic and physical manipulations RNAi • siRNA design • transfection • RNA and protein measurements Assess intended and unintended consequences of mouse ES cells Luciferase measurements siRNA design Microarray analysis Biomaterial Engineering: phage nanowires Experiments Build prototype electrochromic device • Grow iridium nanowires on phage surface • Pattern indium tin oxide slide • Electrodeposit phage nanowires • Overlay solid polymer electrolytes Lab skills Phage material production TEM Fabrication of bio-based device Design and variation of experimental conditions Cite as: Angela Belcher, Drew Endy, Natalie Kuldell and Agi Stachowiak. Course materials for 20.109 Laboratory Fundamentals in Biological Engineering, Fall 2007. MIT OpenCourseWare (http://ocw.mit.edu), Massachusetts Institute of Technology. Downloaded on [DD Month YYYY]. 2 State-of-the-Art Laboratory Classroom Space for 12 students • Demonstration Area • 20 Computer Drops • Gas/Vacuum/Air/Water • Modern Equipment • Cell and Tissue Culture Facility HEPA Filtered Air • 3 Sterile Hoods • 6 incubators • 2 inverted microscopes • Modern Culture Equipment Support Room A u to c la v e • D is h w a s h e r • Ic e M a c h in e • CO2 Source •V acuum Source Cite as: Angela Belcher, Drew Endy, Natalie Kuldell and Agi Stachowiak. Course materials for 20.109 Laboratory Fundamentals in Biological Engineering, Fall 2007. MIT OpenCourseWare (http://ocw.mit.edu), Massachusetts Institute of Technology. Downloaded on [DD Month YYYY]. 3 Course Details Lecture T uesdays and Thursdays 11-12 Lab T uesdays and Thursdays 1-5 W ednesdays and Fridays 1-5 T here are no “make-up” labs Work must be turned in on time l ab reports, homework: at beginning of lab l ab notebook pages: at end of lab You will perform experiments in pairs Assignments can be worked on together but submitted individually Grading 50% Written Work Modules 1 and 2 30% Oral Presentations M odules (1 or 2) and 3 10% Homework Assignments 5% Daily Lab Quizzes 5% Lab Notebooks Foundations/Skills • Basic Laboratory Skills f ollowing and designing protocols fi rst-hand experience with equipment and procedures h ow to keep a lab notebook • Robust Quantitative Analysis of Data s tatistical analysis when appropriate r epetition of protocols to assess quality of findings e ffect of experimental perturbations on outcome • Verbal and Written Communication t wo oral presentations two written reports • Critical Thinking A nalysis and discussion of primary scientific literature Cite as: Angela Belcher, Drew Endy, Natalie Kuldell and Agi Stachowiak. Course materials for 20.109 Laboratory Fundamentals in Biological Engineering, Fall 2007. MIT OpenCourseWare (http://ocw.mit.edu), 4 Massachusetts Institute of Technology. Downloaded on [DD Month YYYY]. ...
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This note was uploaded on 11/11/2011 for the course BIO 20.010j taught by Professor Lindagriffith during the Spring '06 term at MIT.

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