ps2 (1) - Systems Microbiology 1.084J/20.106J PROBLEM SET#2...

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Systems Microbiology 1.084J/20.106J PROBLEM SET #2 – Due Monday Oct. 2 nd Problem 2.1 a. What does proton motive force mean, and why is it important in biology? (Ch.5 Review Question [RQ] 14) b. How is rotational energy in the ATPase used to produce ATP? (Ch.5 RQ 15) Problem 2.2 a. What are the differences in electron donor and carbon source used by Esherichia coli and Acidithiobacillus thioparus (a sulfur chemolithotroph)? (Ch.5 RQ 19) b. The following is a series of coupled electron donors and electron acceptors. Using Figure 5.9, order this series from most energy-yielding to least energy-yielding. (Ch.5 RQ 9) H 2 /Fe 3+ H 2 S/O 2 CH 3 OH/NO 3- H 2 /O 2 Fe 2+ /O 2 NO 2- /Fe 3+ H 2 S/NO 3- c. Explain the circumstances under which the same substance can be either an electron donor or an electron acceptor. d. Consider the following reaction: NADH + fumarate succinate + NAD + + H + a. Using Table 1 calculate the Δ E o ' of the reaction. What is the Δ G o '? Does this reaction produce or consume energy? b. Does this look to you like a potential reaction in a respiratory pathway? Why, or why not? Problem 2.3 a. Explain the following observation: cells of E. coli fermenting glucose grow faster when NO3- is suppled to the culture, and then grow even faster when the culture is highly aerated. (Ch.5 Application Question 4) b.
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