sec_orderbinding

sec_orderbinding - complex. 0 = + k * [ A ] * [ B ] k * [ A...

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20.180:SecondOrderBinding Second Order Binding (of two things) Givens: A physical interaction between molecules A and B . A system that contains some initial concentration of molecules A and B ( A 0 and B 0 , respectively). Tasks: Compute the steady state concentrations of free A , free B , and the A:B complex. Approach: Write differential equation for change in A:B over time. Solve equation at steady state (that is, no change in concentration of the A:B
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Unformatted text preview: complex. 0 = + k * [ A ] * [ B ] k * [ A : B ] on off Solve for K D , the dissociation constant. Equation 1: Note constraints on system due to conservation of mass. Equation 2: [ A ] = [ A ] + [ A : B ] Equation 3: [ B ] = [ B ] + [ A : B ] Note system of three unknowns with three equations (1-3 above)! Solve for unknowns A , B , and A:B (takes you through a quadratic)....
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