exam_2_prep_sol

exam_2_prep_sol - 20.320 Exam 2 Review Problem Solutions 1....

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
20.320 Exam 2 Review Problem Solutions April 20, 2006 1. No inhibition max max max [] 0.5 0.5 0.5[ ] [ ] M M M M vS v KS v S SK = + = + += = Competitive max max max , 1[ ] 0.5 ] 0.5 1 0.5[ ] [ ] ] 1 M I M I M I M I MC I M I v I K v I K I S K I K I KK K = ⎛⎞ ++ ⎜⎟ ⎝⎠ = = =+ Non-Competitive max max max , 1 1 0.5 1 0 . 5 0 . 5 [] [] 0.5 0.5[ ] I M I M I M M MN I M I K v I K v I K S + = + + = + + = =
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
20.320 Exam 2 Review Problem Solutions April 20, 2006 2. a. () 23 0 223 12 1 3 2 3 12 2 3 1 3 2 3 [] M cat kk S E kk k v kk kk S kk k k K k k −− ++ = + = = b. 1) Assume k 3 >> k 2 2) k -2 Æ 0 (irreversible) 3. EC50 would likely be greater for a competitive inhibitor because they have to compete with the substrate whereas a non-competitive inhibitor does not. 4. a. True b. False 5. 0 0 AV R L N ρ ± 6. a. 1) degradation 2) recycling b. 1) degradation 2) trancytosis 7. a. 43 ,, , 10 10 10 DAB DA UB KK K 7 =⋅= = b. 7 , 2 , 5 , 10 10 10 UA DB K K K == = c. 4 , 2 2 , 10 1 0 10 eff K A K =
Background image of page 2
20.320 Exam 2 Review Problem Solutions April 20, 2006 d. , 1 UA K e. K D,AB increases (lower binding affinity) if linker is shorter than needed, and also if linker is long and very flexible. K D,AB decreases (higher binding affinity) if linker is rigid and "just the right length" to reach the 2 nd binding site. 8. d a. 0 20 40 60 80 100 120 140 160 0 0.0005 0.001 0.0015
Background image of page 3
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 11/11/2011 for the course BIO 2.797j taught by Professor Matthewlang during the Fall '06 term at MIT.

Page1 / 3

exam_2_prep_sol - 20.320 Exam 2 Review Problem Solutions 1....

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online