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exam2_practice - 20.320 Spring 2006 Exam 2 Review Problems...

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20 .320 Spring 2006 Exam 2 Review Problems 1) How does K m change with increasing [I] for an competitive inhibitor? For an non- competitive inhibitor? 2) The Briggs-Haldane treatment of a single-substrate, enzyme-catalyzed reaction is as follows: k 1 k 2 k 3 E + S <----> ES <----> EP <----> E + P k- 1 k- 2 k- 3 a. Derive an expression for v = k 3 [EP]. What is K m and k cat ? b. What assumptions do we need to make in order to obtain the Michaelis-Menten K m and k cat expressions from the ones you found in part (a)? 3) Would EC50 likely be higher for a competitive or a non-competitive inhibitor? + 4) A tethered ligand binding to a tethered receptor goes through the following bulk reaction: a. T/F: According to Jencks Theory, the enthalpy of the bulk binding is equal to the sum of the enthalpy of binding at site A and the enthalpy of binding at site B. b. T/F: According to Jencks Theory, the entropy of the bulk binding is equal to the sum of the entropy of binding at site A and the entropy of binding at site B. 5)
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This note was uploaded on 11/11/2011 for the course BIO 2.797j taught by Professor Matthewlang during the Fall '06 term at MIT.

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exam2_practice - 20.320 Spring 2006 Exam 2 Review Problems...

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