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Unformatted text preview: versus unpopulated, kinetic significance iii) The kinetics of catalyzed versus uncatalyzed reactions c) Enzymatic versus non-enzymatic catalysis i) Kinetic analysis: partition experiments, the rate-determining step ii) Chemistry: reactive amino acid residues in active sites iii) Relationships among binding, specificity and rate of enzyme-catalyzed reactions 2) Detecting and exploiting physical interactions a) Interaction forces between molecules b) Canonical structures in proteins c) Assays of physical properties i) Binding equilibria and concentration ii) Turnover iii) Molecular weight of native and denatured proteins iv) Fluorescence and FRET. 3) Biological implications a) Allosteric interactions b) Specific versus non-specific binding and distinguishing functional binding interactions c) High energy compounds: synthesis and hydrolysis d) Catalysis of vectorial reactions: motor functions, protein folding, pumps....
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- Spring '08