lec11_walt_shen

lec11_walt_shen - Asymmetric Numb Distribution is Critical...

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Unformatted text preview: Asymmetric Numb Distribution is Critical for Asymmetric Cell Division of Mouse cerebral cortical stem cells and neuroblasts by: Qin Shen, Weimin Zhong, Yuh Nung Jan, & Sally Temple Tony Walters 9.18 March 15 2005 Background Few cells make many different types of cells. E10 mice only have single layer of proliferating cells Understand the mechanisms that allow diversification Understand normal neural development Repair damaged nervous system Invertebrate model Numb causes asymmetric divisions in both PNS and CNS Loss of Numb function produces identical daughter cells However Numb does not confer a specific fate Fate lineage trees of mice cells are similar to those of Drosophila Evolutionarily conserved mechanism Vertebrate Models Numb homologue found in chicken, rat, mouse, human Mouse Numb can rescue fly Numb mutant Contradictory findings Over expression in vivo in chick CNS cause progenitor proliferation Over expression in vitro leads to increased neuronal production Multiple roles of numb during development? Asymmetric Numb distributions are found, but does this lead to different cell fates? Methods Mouse embryos E10-E14 Used low density cultures to watch cell division Video Microscopy Used immunohistochemistry to show Numb, Nestin, Beta-Tubulin-III, LeX Morphological measurements process number, lengths, branch points, etc. Results Asymmetric Numb distribution is necessary for Asymmetric cell division Numb distribution changes at different developmental stages Numb levels influence morphology of symmetrical cell pairs Numb expression...
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lec11_walt_shen - Asymmetric Numb Distribution is Critical...

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