MIT1_018JF09_Lec14_chemo

MIT1_018JF09_Lec14_chemo - 1.018 7.30J Population Growth in...

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1.018 – 7.30J Population Growth in Chemostats – Lecture Notes Steady State, resource-limited growth Jaques Monod (1950s) Wanted to look at the effects of a limited nutrient on the growth rate of bacteria Noticed . . . [S 3 ] [S 2 ] [S] = Substrate (Nutrient) ln N Concentration [S 1 ] Slope = r (growth rate) Time Hypothesized . . . S K S r r s + = max r max r But could not get good data here. K s [S] Needed system with constant low supply of nutrients Chisholm
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1.018 – 7.30J Chemostat Theory System in which a fresh supply of nutrients is fed to a culture of constant volume at a fixed rate, and the contents of the culture are withdrawn at the same rate. S i , f S,f S,N, Q Assumptions - Completely Mixed - Inflow = Outflow - One limiting Nutrient in Influent Medium Units cells /L L L/hr μ g/L μ g/L μ g/cell cells/ μ g hr -1 hr Constants & Variables N = Cell Concentration V = Volume of Culture f = Flow Rate S i = Influent Limiting Nutrient Concentration S = Residual Concentration in Vessel Q = Cell Quota Y = Yield Coefficient = 1/Q Dilution Rate D = f/V Residence Time = 1/D
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1.018 – 7.30J
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This note was uploaded on 11/11/2011 for the course BIO 7.344 taught by Professor Bobsauer during the Spring '08 term at MIT.

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MIT1_018JF09_Lec14_chemo - 1.018 7.30J Population Growth in...

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