ses2_slides

ses2_slides - MIT OpenCourseWare http:/ocw.mit.edu 7.344...

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MIT OpenCourseWare http://ocw.mit.edu 7.344 Directed Evolution: Engineering Biocatalysts Spring 2008 For information about citing these materials or our Terms of Use, visit: http://ocw.mit.edu/terms .
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Library generation by point mutation Cadwell, R.C.; Joyce, G.F. Randomization of genes by PCR mutagenesis. PCR Methods Applic. 1992 , 2 , 28-33. Kegler-Ebo, D.M.; Docktor, C.M.; DiMaio, D. Codon cassette mutagenesis: a general method to insert or replace individual codons by using universal cassettes. Nuc. Acids Res. 1994 , 22(9), 1593-1599.
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PCR mutagenesis • What is the method? What are the steps by which it is conducted? • What are the results? • What is mutational bias? Why is it a problem in library construction? • What are the benefits to using this method? • What are the pitfalls?
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Watson-Crick base pairing
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Guanosine tetraplex Image removed due to copyright restrictions. Please see http://www.imb- jena.de/images/groups/suehnel/suehnel_website_ 2b_big.gif .
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H-DNA Image of triple-stranded DNA removed due to copyright restrictions.
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ses2_slides - MIT OpenCourseWare http:/ocw.mit.edu 7.344...

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