{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

ses3_ln - MIT OpenCourseWare http/ocw.mit.edu 7.344...

Info icon This preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
MIT OpenCourseWare http://ocw.mit.edu 7.344 Directed Evolution: Engineering Biocatalysts Spring 2008 For information about citing these materials or our Terms of Use, visit: http://ocw.mit.edu/terms .
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Session 3 Lecture Notes 1. DNA Shuffling – what is the method and what are the steps? Look at Figure 1. Starts with a group of enzymes that are similar or are point mutants of one another. Allows for the beneficial parts of similar enzymes to be recombined. 2. What are the results? New lactamase variants were discovered that increased the MIC of cefotaxime from 0.02 μ g per ml to 640 μ g per ml – much better at hydrolyzing the antibiotic! 3. How does this technique compare with others? EP-PCR (Alone) gave MIC of 0.32 μ g. 4. What are the benefits to using this method? More mutations occur in each clone potentially leading to greater changes in enzymatic activity. 5. What are the pitfalls? The process is a bit lengthy. 6. StEP extension – what is the method and what are the steps? It is a
Image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern