ses3_ln

ses3_ln - MIT OpenCourseWare http:/ocw.mit.edu 7.344...

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MIT OpenCourseWare http://ocw.mit.edu 7.344 Directed Evolution: Engineering Biocatalysts Spring 2008 For information about citing these materials or our Terms of Use, visit: http://ocw.mit.edu/terms .
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Session 3 Lecture Notes 1. DNA Shuffling – what is the method and what are the steps? Look at Figure 1. Starts with a group of enzymes that are similar or are point mutants of one another. Allows for the beneficial parts of similar enzymes to be recombined. 2. What are the results? New lactamase variants were discovered that increased the MIC of cefotaxime from 0.02 μ g per ml to 640 μ g per ml – much better at hydrolyzing the antibiotic! 3. How does this technique compare with others? EP-PCR (Alone) gave MIC of 0.32 μ g. 4. What are the benefits to using this method? More mutations occur in each clone potentially leading to greater changes in enzymatic activity. 5. What are the pitfalls? The process is a bit lengthy. 6.
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This note was uploaded on 11/11/2011 for the course BIO 7.344 taught by Professor Bobsauer during the Spring '08 term at MIT.

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ses3_ln - MIT OpenCourseWare http:/ocw.mit.edu 7.344...

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