Library generation by recombinationStemmer, W.P.C. Rapid evolution of a protein in vitro by DNA shuffling. Nature1994, 370, 389-391.Zhao, H.; Giver, L.; Shao, Z.; Affholter, J. A.; Arnold, F. A. Molecular evolution by staggered extension process (StEP) in vitro recombination. Nature Biotechnol.1998, 16, 258-261.
DNA shuffling•What is the method? What are the steps by which it is conducted?•What are the results?•How does it compare with other methods?•What are the benefits to using this method?•What are the pitfalls?
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