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ses3_slides - What are the steps by which it is conducted...

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MIT OpenCourseWare http://ocw.mit.edu 7.344 Directed Evolution: Engineering Biocatalysts Spring 2008 For information about citing these materials or our Terms of Use, visit: http://ocw.mit.edu/terms .
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Library generation by recombination Stemmer, W.P.C. Rapid evolution of a protein in vitro by DNA shuffling. Nature 1994 , 370 , 389-391. Zhao, H.; Giver, L.; Shao, Z.; Affholter, J. A.; Arnold, F. A. Molecular evolution by staggered extension process (StEP) in vitro recombination. Nature Biotechnol. 1998 , 16 , 258- 261.
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DNA shuffling What is the method? What are the steps by which it is conducted? What are the results? How does it compare with other methods? What are the benefits to using this method? What are the pitfalls?
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