ses3_slides

ses3_slides - What are the steps by which it is conducted?...

Info iconThis preview shows pages 1–10. Sign up to view the full content.

View Full Document Right Arrow Icon
MIT OpenCourseWare http://ocw.mit.edu 7.344 Directed Evolution: Engineering Biocatalysts Spring 2008 For information about citing these materials or our Terms of Use, visit: http://ocw.mit.edu/terms .
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Library generation by recombination Stemmer, W.P.C. Rapid evolution of a protein in vitro by DNA shuffling. Nature 1994 , 370 , 389-391. Zhao, H.; Giver, L.; Shao, Z.; Affholter, J. A.; Arnold, F. A. Molecular evolution by staggered extension process (StEP) in vitro recombination. Nature Biotechnol. 1998 , 16 , 258- 261.
Background image of page 2
DNA shuffling • What is the method? What are the steps by which it is conducted? • What are the results? • How does it compare with other methods? • What are the benefits to using this method? • What are the pitfalls?
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
DNA shuffling Parent sequences DNase I enzyme Random fragments PCR without primers Shuffled Product Denaturization Fragment Annealing Extension via Polymerase
Background image of page 4
StEP (Staggered extension process) • What is the method?
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 6
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 8
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 10
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: What are the steps by which it is conducted? • What are the results? • What are the benefits to using this method? • What are the pitfalls? StEP Image removed due to copyright restrictions. Please see Fig. 1 in Zhao, H., L. Giver, A. Shao, J. A. Affholter, and F. A. Arnold. “Molecular evolution by staggered extension process (StEP) in vitro recombination.” Nature Biotechnol. 16(1998): 258-261. For next week… • Zone of clearing assay • Western blot • Antibody structure/production • Intro to phage display selection Antibody structure and function Diagram of antibody removed due to copyright restrictions. scFv Intro to phage display pVIII pVII + pIX ssDNA pIII pVI M13 phage particle Phagemid Image removed due to copyright restrictions. Please see Fig. 2 in <http://www.biology-medicine.com/Technical-Articles/Molecular-Biology/2008-01-11/90.html>...
View Full Document

This note was uploaded on 11/11/2011 for the course BIO 7.344 taught by Professor Bobsauer during the Spring '08 term at MIT.

Page1 / 10

ses3_slides - What are the steps by which it is conducted?...

This preview shows document pages 1 - 10. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online