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ses4_slides - MIT OpenCourseWare http/ocw.mit.edu 7.344...

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MIT OpenCourseWare http://ocw.mit.edu 7.344 Directed Evolution: Engineering Biocatalysts Spring 2008 For information about citing these materials or our Terms of Use, visit: http://ocw.mit.edu/terms .
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Alternative methods for library generation Lai, Y.-P.; Huang, J.; Wang, L.-F.; Li, J.; Wu, Z.-J. A new approach to random mutagenesis in vitro . Biotech. Bioeng. 2004 , 86 , 622-627. Coia, G.; Ayres, A.; Lilley, G.G.; Hudson, P.J.; Irving, R.A. Use of mutator cells as a means for increasing production levels of a recombinant antibody directed against Hepatitis B. Gene 1997 , 201 , 203-209.
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Chemical mutagenesis How does mutagenesis occur? What do the authors use this method to study? What are the results? What are the benefits to using this method? Are there any pitfalls to this method? S O O O H 3 C EMS
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