ses5_slides

ses5_slides - MIT OpenCourseWare http:/ocw.mit.edu 7.344...

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MIT OpenCourseWare http://ocw.mit.edu 7.344 Directed Evolution: Engineering Biocatalysts Spring 2008 For information about citing these materials or our Terms of Use, visit: http://ocw.mit.edu/terms .
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Enzyme evolution by genetic complementation Yano, T.; Oue, S.; Kagamiyama, H. Directed evolution of an aspartate aminotransferase with new substrate specificities. Proc. Natl. Acad. Sci. USA 1998 , 95 , 5511-5515. Otten, L.G.; Sio, C.F.; Vrielink, J.; Cool, R.H.; Quax, W.J. Altering the substrate specificity of cephalosporin acylase by directed evolution of the β -subunit. J. Biol. Chem. 2002 , 277 (44) , 42121-42127.
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aminotransferase • What are the authors trying to do? • What is the process by which they conduct their evolution? Explain the Materials and Methods section. • What method of library generation do they use? Is this a good choice? • How do they carry out their selection? • What are the results?
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ses5_slides - MIT OpenCourseWare http:/ocw.mit.edu 7.344...

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