ses6_slides

ses6_slides - MIT OpenCourseWare http:/ocw.mit.edu 7.344...

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MIT OpenCourseWare http://ocw.mit.edu 7.344 Directed Evolution: Engineering Biocatalysts Spring 2008 For information about citing these materials or our Terms of Use, visit: http://ocw.mit.edu/terms .
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Enzyme evolution by chemical complementation Baker, K.; Bleczinski, C.; Lin, H.; Salazar-Jimenez, G.; Sengupta, D.; Krane, S.; Cornish, V.W. Chemical complementation: a reaction- independent genetic assay for enzyme catalysis. Proc. Natl. Acad. Sci. USA 2002 , 99 (26), 16537-16542. Azizi, B.; Chang, E.I.; Doyle, D.F. Chemical complementation: small- molecule-based genetic selection in yeast. Biochem. Biophys. Res. Commun. 2003 , 306 , 774-780. Lin, H.; Tao, H.; Cornish, V.W. Directed evolution of a glycosynthase via chemical complementation. J. Am. Chem. Soc. 2004 , 126 , 15051-15059.
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developed in the Cornish lab • What are the authors trying to do? • How do they go about engineering their system of complementation? • What enzyme do they use to test their system? How does this work? (Figure 2, panel B) • What are the results? (Figure 3, panels B and C) How do the authors confirm that the change in transcription is caused by enzyme turnover? • Is this strategy general?
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This note was uploaded on 11/11/2011 for the course BIO 7.344 taught by Professor Bobsauer during the Spring '08 term at MIT.

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ses6_slides - MIT OpenCourseWare http:/ocw.mit.edu 7.344...

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