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MIT OpenCourseWare 7.344 Directed Evolution: Engineering Biocatalysts Spring 2008 For information about citing these materials or our Terms of Use, visit: .
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Session 8 Lecture Notes 1. Debrief on Codon Devices next week since we have 3 papers this week. 2. Remind everyone about the assignment coming up due Session 10. People should have picked out their enzyme by next session and bring choice to class for approval. 3. What has phage display historically been used for? Is this a reasonable application? Briefly get someone to explain the system of superinfection, phage biology etc… 4. The authors are trying to use phage display to evolve a metalloenzyme – specifically a beta lactamase (zinc containing) using catalytic elution. They put forth that this method should be general for any enzyme that requires the presence of a cofactor. 5. The elution works as follows: phage are produced that have a p3 fusion of the metalloenzyme. Any metals are removed from the system by complexation and phage are loaded onto a solid support coated with substrate. Addition of the metal cofactor allows for enzyme turnover and phage are released, since they have more affinity for the substrate than for the product. 6.
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This note was uploaded on 11/11/2011 for the course BIO 7.344 taught by Professor Bobsauer during the Spring '08 term at MIT.

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ses8_ln - MIT OpenCourseWare http:/ 7.344...

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