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MIT OpenCourseWare http://ocw.mit.edu 7.344 Directed Evolution: Engineering Biocatalysts Spring 2008 For information about citing these materials or our Terms of Use, visit: http://ocw.mit.edu/terms .
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Enzyme evolution using phage display Ponsard, I.; Galleni, M.; Soumillion, P.; Fastrez, J. Selection of metalloenzymes by catalytic activity using phage display and catalytic elution. Chembiochem 2001 , 2 253-259. Strobel, H.; Ladant, D.; Jestin, J.L. In vitro selection for enzymatic activity: a model study using adenylate cyclase. J. Mol. Biol. 2003 , 332 , 1-7. Pedersen, H.; Holder, S.; Sutherlin, D.P.; Schwitter, U.; King, D.S.; Schultz, P.G. A method for the directed evolution and functional cloning of enzymes. Proc. Natl. Acad. Sci. USA 1998 , 95 , 10523-10528.
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Phage display: the basics Phagemid Image removed due to copyright restrictions. Please see: Fig. 2 in <http://www.biology- medicine.com/Technical-Articles/Molecular- Biology/2008-01-11/90.html> M13 phage particle pVIII pVII + pIX ssDNA pIII pVI
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Enzyme evolution using phage display and catalytic elution • What are the authors trying to do? • How does catalytic elution work?
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