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ses9_slides - What are the benefits and pitfalls of this...

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MIT OpenCourseWare http://ocw.mit.edu 7.344 Directed Evolution: Engineering Biocatalysts Spring 2008 For information about citing these materials or our Terms of Use, visit: http://ocw.mit.edu/terms .
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Enzyme evolution by bacterial cell surface display Olsen, M.J.; Stephens, D.; Griffiths, D.; Daugherty, P.; Georgiou, G.; Iverson, B.L. Function-based isolation of novel enzymes from a large library. Nat. Biotechnol. 2000 , 18 , 1071-1074. Kim, Y.-S.; Jung, H.-C.; Pan, J.-G. Bacterial cell surface display of an enzyme library for selective screening of improved cellulose variants. App. Environ. Microbiol. 2000 , 66(2) , 788-793.
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OmpT evolution • What are the authors trying to do? • How is the library formed any why do the authors chose this method? • What is FRET? What is FACS? • What are the results presented?
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Unformatted text preview: What are the benefits and pitfalls of this method? Bacterial surface display and screening of OmpT Image removed due to copyright restrictions. Please see Fig. 1 in Olsen, M. J., D. Stephens, D. Griffiths, P. Daugherty, G. Georgiou, and B. L. Iverson. Function-based isolation of novel enzymes from a large library. Nat. Biotechnol. 18(2000):1071-1074. What is FRET? Image removed due to copyright restrictions. Please see Fig. 2 in Olsen, M. J., D. Stephens, D. Griffiths, P. Daugherty, G. Georgiou, and B. L. Iverson. Function-based isolation of novel enzymes from a large library. Nat. Biotechnol. 18(2000):1071-1074. What is FACS? Diagram of Fluorescent-Activated Cell Sorting (FACS) removed due to copyright restrictions....
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This note was uploaded on 11/11/2011 for the course BIO 7.344 taught by Professor Bobsauer during the Spring '08 term at MIT.

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ses9_slides - What are the benefits and pitfalls of this...

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