ses10_ln

ses10_ln - MIT OpenCourseWare http:/ocw.mit.edu 7.344...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
MIT OpenCourseWare http://ocw.mit.edu 7.344 Directed Evolution: Engineering Biocatalysts Spring 2008 For information about citing these materials or our Terms of Use, visit: http://ocw.mit.edu/terms .
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Session 10 Lecture Notes 1. Why engineer antibodies? The authors discuss this a bit – what do you think? Implications for cancer biology. What are the limitations the authors identify with previous display methods that make their system attractive? Expression bias in e.coli – phage display requires soluble expression of a protein for incorporation as a fusion with a phage coat protein – problem for disulfide-bond containing proteins. Phage display may also select for alternative properties of enzymes including reduced host toxicity or increased phage infectivity. Major advantage for cell-based methods is the total number of surface fusions – as many as thousands and can be screened by FACS – a very fast method. One problem, steric interference of the cell wall glycans may prevent substrate binding. 2.
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 11/11/2011 for the course BIO 7.344 taught by Professor Bobsauer during the Spring '08 term at MIT.

Page1 / 3

ses10_ln - MIT OpenCourseWare http:/ocw.mit.edu 7.344...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online