ses11_slides

Ses11_slides - MIT OpenCourseWare http/ocw.mit.edu 7.344 Directed Evolution Engineering Biocatalysts Spring 2008 For information about citing these

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MIT OpenCourseWare http://ocw.mit.edu 7.344 Directed Evolution: Engineering Biocatalysts Spring 2008 For information about citing these materials or our Terms of Use, visit: http://ocw.mit.edu/terms .
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Enzyme evolution using ribosome display Amstutz, P.; Pelletier, J. N.; Guggisberg, A.; Jermutus, L.; Cesaro- Tadic, S.; Zahnd, C.; Pluckthun, A. In Vitro Selection for Catalytic Activity with Ribosome Display J. Am. Chem. Soc. 2002 , 124(32), 9396-9403. Takahashi, F.; Funabashi, H.; Mie, M.; Endo, Y.; Sawasaki, T.; Aizawa, M.; Kobatake, E. Activity-based in vitro selection of T4 DNA ligase. Biochem. Biophys. Res. Commun. 2005 , 336 , 987-993.
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β -lactamases using catalytic activity • What limitations do the authors identify with in vivo or “partially” in vitro type selections? • What is the strategy used by the authors? Does everyone understand IVT? • What is the library size limitation? • What sort of controls do the authors do to test
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This note was uploaded on 11/11/2011 for the course BIO 7.344 taught by Professor Bobsauer during the Spring '08 term at MIT.

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Ses11_slides - MIT OpenCourseWare http/ocw.mit.edu 7.344 Directed Evolution Engineering Biocatalysts Spring 2008 For information about citing these

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