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ses11_slides - MIT OpenCourseWare http/ocw.mit.edu 7.344...

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MIT OpenCourseWare http://ocw.mit.edu 7.344 Directed Evolution: Engineering Biocatalysts Spring 2008 For information about citing these materials or our Terms of Use, visit: http://ocw.mit.edu/terms .
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Enzyme evolution using ribosome display Amstutz, P.; Pelletier, J. N.; Guggisberg, A.; Jermutus, L.; Cesaro- Tadic, S.; Zahnd, C.; Pluckthun, A. In Vitro Selection for Catalytic Activity with Ribosome Display J. Am. Chem. Soc. 2002 , 124(32), 9396-9403. Takahashi, F.; Funabashi, H.; Mie, M.; Endo, Y.; Sawasaki, T.; Aizawa, M.; Kobatake, E. Activity-based in vitro selection of T4 DNA ligase. Biochem. Biophys. Res. Commun. 2005 , 336 , 987-993.
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Selection of β -lactamases using catalytic activity What limitations do the authors identify with in vivo or “partially” in vitro type selections? What is the strategy used by the authors? Does everyone understand IVT? What is the library size limitation? What sort of controls do the authors do to test their strategy? What are the results?
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