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MIT OpenCourseWare 7.344 Directed Evolution: Engineering Biocatalysts Spring 2008 For information about citing these materials or our Terms of Use, visit: .
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Session 12 Lecture Notes 1. The authors have developed a new strategy to link genotype and phenotype based on compartmentalization. Essentially is like ribosome display but prevents the crosstalk we identified as a problem last week. A protein and the gene that encodes it are encapsulated within an emulsion along with substrate that is somehow detectable. The key in this first paper is that the gene is the substrate. How do the authors prevent crosstalk here before emulsification? Is this reasonable? Where is the data?! 2. The compartments are made by adding an aqueous reaction mixture to a stirring solution of mineral oil containing surfactants. Droplets are 2.6 um in diameter – about the size of bacterial cells. The volume of a droplet is about 5 femtoliters. One mL of the emulsion contains about 10 10 compartments (or genes). How are the emulsions broken up – ether? Does everyone
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ses12_ln - MIT OpenCourseWare http:/ 7.344...

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