ses12_slides

ses12_slides - MIT OpenCourseWare http:/ocw.mit.edu 7.344...

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MIT OpenCourseWare http://ocw.mit.edu 7.344 Directed Evolution: Engineering Biocatalysts Spring 2008 For information about citing these materials or our Terms of Use, visit: http://ocw.mit.edu/terms .
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Enzyme evolution using in vitro compartmentalization Tawfik, D.S.; Griffiths, A.D. Man-made cell-like compartments for molecular evolution. Nat. Biotechnol. 1998 , 16 , 652-656. Griffiths, A.D.; Tawfik, D.S. Directed evolution of an extremely fast phosphotriesterase by in vitro compartmentalization. EMBO J. 2003 , 22 , 24-35.
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In vitro compartmentalization • What is the strategy presented by the authors for linking genotype and phenotype? (Figure 1, A) • How are the compartments made? How big are they? What is the ratio of reaction volume to library size? • What is the selection method? • What sort of controls do the authors do to test their strategy? • What are the results? • What are the problems with this strategy?
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Image removed due to copyright restrictions. Please see Fig. 1 in Tawfik, D.S., and A. D.
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This note was uploaded on 11/11/2011 for the course BIO 7.344 taught by Professor Bobsauer during the Spring '08 term at MIT.

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ses12_slides - MIT OpenCourseWare http:/ocw.mit.edu 7.344...

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