Alternate methods for enzyme designHill, C. M.; Li, W.-S.; Thoden, J. B.; Holden, H. M.; Raushel, F. M. Enhanced Degradation of Chemical Warfare Agents through Molecular Engineering of the Phosphotriesterase Active Site J. Am. Chem. Soc.2003, 125(30), 8990-8991.Williams, G.J.; Woodhall, T.; Nelson, A.; Berry, A. Structure-guided saturation mutagenesis of N-acetylneuraminic acid lyase for the synthesis of sialic acid mimetics. Prot. Eng. Des. Select.2005, 18(5), 239-246.Reetz, M.T.; Bocola, M.; Carballeira, J.D.; Zha, D.; Vogel, A. Expanding the range of substrate acceptance of enzymes: Combinatorial active-site saturation test. Angew. Chem. Int. Ed.2005, 44, 4192-4196.
Molecular engineering of PTE•What is the PTE and what are its substrates? What is the mechanism for substrate degradation and what residues are important?•What is the mutagenesis method used? Why do the authors choose this method?•How are the active mutants recovered?•What are the results?
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