ses13_slides

ses13_slides - MIT OpenCourseWare http:/ocw.mit.edu 7.344...

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MIT OpenCourseWare http://ocw.mit.edu 7.344 Directed Evolution: Engineering Biocatalysts Spring 2008 For information about citing these materials or our Terms of Use, visit: http://ocw.mit.edu/terms .
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Alternate methods for enzyme design Hill, C. M.; Li, W.-S.; Thoden, J. B.; Holden, H. M.; Raushel, F. M. Enhanced Degradation of Chemical Warfare Agents through Molecular Engineering of the Phosphotriesterase Active Site J. Am. Chem. Soc. 2003 , 125(30) , 8990-8991. Williams, G.J.; Woodhall, T.; Nelson, A.; Berry, A. Structure-guided saturation mutagenesis of N-acetylneuraminic acid lyase for the synthesis of sialic acid mimetics. Prot. Eng. Des. Select. 2005 , 18(5) , 239-246. Reetz, M.T.; Bocola, M.; Carballeira, J.D.; Zha, D.; Vogel, A. Expanding the range of substrate acceptance of enzymes: Combinatorial active-site saturation test. Angew. Chem. Int. Ed. 2005 , 44 , 4192-4196.
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• What is the PTE and what are its substrates? What is the mechanism for substrate degradation and what residues are important? • What is the mutagenesis method used? Why do the authors choose this method? • How are the active mutants recovered? • What are the results?
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This note was uploaded on 11/11/2011 for the course BIO 7.344 taught by Professor Bobsauer during the Spring '08 term at MIT.

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ses13_slides - MIT OpenCourseWare http:/ocw.mit.edu 7.344...

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