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Unformatted text preview: water-soluble protein (this electronic transition is associated with aromatic transitions) as a function of time as it is degraded by an enzyme that destroys aromatic molecules. The kinetics of the degradation reaction are known to be exceedingly fast. 4-5. You are in the lab and you make absorbance measurements of an analyte at a series of concentrations ranging from 0.08 absorbance units to 1.95 absorbance units (10 different concentrations). You then measure the absorbance of two unknowns and find the absorbance values to be 0.05 and 5.00. Your lab mate tells you that there is something wrong with just using the linear least squares equation from the calibration curve and placing the absorbance values of the unknowns in the equation to find the concentrations of the two unknowns. Why is this? What are all of the possibilities? How would you correct these situations?...
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This note was uploaded on 11/12/2011 for the course CHEM 2001 taught by Professor Staff during the Fall '08 term at LSU.
- Fall '08