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Homework 4 SP 2008

Homework 4 SP 2008 - water-soluble protein(this electronic...

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Homework 4 – Chemistry 2001 SP 2008 This is due Wednesday, February 20, 2008 (Lecture 9) DO NOT TURN IN THIS SHEET Make sure that you carefully set up your thesis sentence and then have your defending statements written in a clear fashion so as to support the thesis sentence of your paragraph explanations in all essay question responses. PLEASE PLACE YOUR GROUP NAME ON YOUR HOMEWORK ALONG WITH YOUR NAME 4-1. Explain why it is that molecules have broad absorption spectra. 4-2. Why is it that a double-beam instrument gives “better” data? That is, why is it that a double- beam instrument gives better responses than a single-beam instrument? 4-3. Draw a block diagram for a simple, fluorescence spectrometer. Make sure to label all components (do not use acronyms). Note: you should be able to do this for all other spectral techniques we have discussed in this section of material. 4-4. What kind of source, wavelength selector, solvent, cuvette and detector would you choose for the following experiment. You wish to measure the absorbance of a band at 280 nm for a
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Unformatted text preview: water-soluble protein (this electronic transition is associated with aromatic transitions) as a function of time as it is degraded by an enzyme that destroys aromatic molecules. The kinetics of the degradation reaction are known to be exceedingly fast. 4-5. You are in the lab and you make absorbance measurements of an analyte at a series of concentrations ranging from 0.08 absorbance units to 1.95 absorbance units (10 different concentrations). You then measure the absorbance of two unknowns and find the absorbance values to be 0.05 and 5.00. Your lab mate tells you that there is something wrong with just using the linear least squares equation from the calibration curve and placing the absorbance values of the unknowns in the equation to find the concentrations of the two unknowns. Why is this? What are all of the possibilities? How would you correct these situations?...
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