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508lecture1 - Lecture#1 Administrative notes No required...

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Administrative notes: No required textbook, readings will come from the primary literature Exams will be held Wednesday evenings from 7-9pm. Material from “Techniques and Discussion” sections will be covered on exams. Biochemistry inspires SHOCK and AWE! Lecture 1 2/4/04 What is inside the cell? - What are the concentrations of proteins and small molecules? - What are the differences between eukaryotic and prokaryotic cells? - How does studying something in vitro relate to reality inside a cell? We need to know the sizes of cells and their constituents in order to understand the “jungle” of metabolism. How do these intracellular activities influence metabolic pathways and regulatory mechanisms by altering the thermodynamic and kinetic properties of macromolecular interactions? Definition of BIOCHEMISTRY: Understand in molecular detail (quantitatively) the function of some cellular machinery in vitro (under controlled conditions) AND to relate this information to what occurs in reality inside a living cell.
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