508lecture27

508lecture27 - Lecture #27 Lecture 27 4/23/04 Exam 3: Avg....

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Lecture #27 Lecture 27 4/23/04 Exam 3: Avg. 62, grades from 8-87 From Exam question #2 Many mechanisms by which enzymes catalyze reactions Yarus inhibitor= transition state analog for peptide bond formation Mimics tetrahedral intermediate in peptide bond formation Select for RNA that binds tightly to Yarus, it can catalyze peptide bond formation! Supports transition state stabilization theory of enzyme catalysis #3 Correct answer ultimately depends on kinetics and concentrations a) fMet – intitation- goes into the P site (requires energy) b) proofreading: non-cognate discriminates w/out GTP hydrolysis’ near-cognate uses a GTP when proofread out Use 2 GTP, 1 for Ef-Tu, and 1 fro Ef-G For more info on exam 3 see the exam answers and the T&D9 PROTEIN folding and degradation The Small Difference in the free energy of the folded and unfolded states makes it very difficult to predict structure from amino acid sequence. Remember the Levinthol paradox and Anfinsen’s hypothesis from last lecture Anfinsen’s – ( in vitro ) RNase has 4 disulfide bonds- if a thiol isomerase is present, it can
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508lecture27 - Lecture #27 Lecture 27 4/23/04 Exam 3: Avg....

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