{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

508lecture35 - Lecture 35 Review Sunday 1-4pm room 2-147 TD...

Info icon This preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Lecture 35 5/12/04 Review Sunday 1-4pm room 2-147 TD review starts at 2:30 Continued from lecture 34 (see cartoon diagram) Degradation through poklyubiquitination- (eucaryotes) Specificity comes from E3 (remember ~100 E3’s inside the cell) You could imagine one type of E3 would interact with positively charged N-terminal amino acid, and another would recognize hydrophobic N-term. Several ubiquitins are added on to a lysine on the protein by E3 and E2 Ubiquitination may have many roles inside the cell- we are only discussing its role in protein degradation in yeast. Roles of E1,E2,E3 in ubiquitination (See handout 4d for cartoons of all of this) 1) ATP activated Ub through adenylation 2) E1 binds covalently to C-term of Ub through a thioester Need fewer E1-> higher turnover rate than E2, E3 3) Ub transferr to E2, again as a thioester 4) Substrate needs N-end amino acid + lysine-> E3 interacts with substrate and E2 to catalyze attachment oof Ub to lysine through isopeptide linkage 5)
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern