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4106212B - iQ™ SYBR® Green Supermix 100 x 50 µl...

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Unformatted text preview: iQ™ SYBR® Green Supermix 100 x 50 µl reactions 500 x 50 µl reactions 1,000 x 50 µl reactions 2,000 x 50 µl reactions 170-8880 170-8882 170-8884 170-8885 For Research purposes only Store at -20°C Storage and Stability Store the iQ SYBR Green Supermix at -20°C in a constant temperature freezer. Avoid repeated freeze/thaw cycles. When stored under these conditions the supermix is stable for one year after ship date. You may aliquot the supermix and store a portion at 4°C for ready use. At 4°C the supermix is stable for six months. Kit Contents iQ SYBR Green Supermix is a 2X mix for real-time PCR applications containing a proprietary buffer and stabilizers specifically optimized for qPCR using SYBR Green I. This mix provides the highest level of specificity to reduce the occurrence or delay the detection of primer-dimer and other non-specific artifacts. The antibody-mediated hot-start iTaq™ DNA polymerase is activated after an initial three minute denaturation step at 95°C. Reagent Kit Size Volume Description iQ SYBR Green Supermix (Orange Cap) 100 reactions 500 reactions 1,000 reactions 2,000 reactions 1.25 ml x 2 1.25 ml x 10 1.25 ml x 20 50 ml (bottle) 2X reaction buffer with dNTPs, iTaq DNA polymerase, 6 mM MgCl2, SYBR Green I, fluorescein, and stabilizers iQ SYBR Green Supermix comes optimized for real-time PCR applications. It contains fluorescein for experimental plate well factor collection on the iCycler iQ® system. The addition of fluorescein will not affect the reaction efficiency or sensitivity of detection. If you would like to have extra MgCl2, a 50 mM MgCl2 solution is available free of charge upon request. Please request catalog number 170-8872 for 1.25 ml of this solution. Quality Control The iQ SYBR Green Supermix is free of contaminating DNase and RNase. Functionally, the iQ SYBR Green Supermix is tested to demonstrate resolution over six orders of dynamic range of plasmid DNA and three orders of human genomic DNA to detect a single copy gene, IL-1b. 4106212B.qxp 10/9/2007 8:29 AM Page 1 Reaction Set Up Thaw all components at room temperature. Mix vigorously, and the centrifuge to collect contents to the bottom of the tube before using. Component Volume per reaction Final concentration iQ SYBR Green Supermix Primer 1 Primer 2 Sterile water DNA template 25 µl x µl x µl x µl x µl 1X 100 nM–500 nM 100 nM–500 nM Total Volume 50 µl Reagents and Materials Not Supplied Screw cap microcentrifuge tubes Pipette tips, preferably aerosol barrier tips Vortexer Microcentrifuge Optical plates/tubes Sterile water Reaction primers Real-time PCR detection system Recommendations for Optimal Results Due to the sensitivity of quantitative PCR, results can be easily affected by pipetting errors. • • • Always prepare a master mix of iQ SYBR Green Supermix containing the primers and probe. Add the template DNA sample to aliquots of the master mix for optimal reproducibility of replicate samples. This allows you to pipet once into the sample well or tube. Individual pipetting of replicate samples is not recommended. To learn more about Bio-Rad’s complete solution for amplification, visit our website: www.bio-rad.com/amplification NOTICE TO PURCHASER: LIMITED LICENSE Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155, 5,677,152 (claims 1 to 23 only), 5,733,258 (claims 1 and 6 only), 5,407,800, 5,322,770, 5,310,652, 5,994,056, 6,171,785, and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser’s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. Bio-Rad Laboratories, Inc. is licensed by Molecular Probes, Inc. to sell reagents containing SYBR Green I for use in real-time PCR, for research purposes only. iQ is a trademark of Bio-Rad Laboratories. SYBR Green is a registered trademark of Molecular Probes, Inc. Bio-Rad Laboratories, Inc. 2000 Alfred Nobel Drive, Hercules, CA 94547 510-741-1000 4106212 Rev B ...
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