coa_ef0511

coa_ef0511 - Description CERTIFICATE OF ANALYSIS Shrimp...

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CERTIFICATE OF ANALYSIS Shrimp Alkaline Phosphatase (SAP) #EF0511 500 u± Lot: Expiry Date: ± Concentration: 1 u/µl Supplied with: 3 x 1 ml of 10X Reaction Buffer Store at -20°C In total 4 vials. BSA included Description Shrimp Alkaline Phosphatase (SAP) catalyzes the release of 5'- and 3'-phosphate groups from DNA, RNA and nucleotides. This enzyme can also remove phosphate groups from proteins. Applications Dephosphorylation of cloning vector DNA to avoid recircularization. Degradation of dNTPs in PCR mixture prior to sequencing of PCR products (1). Dephosphorylation of DNA and RNA 5'-ends prior to labelling with T4 Polynucleotide Kinase. Dephosphorylation of DNA and RNA (2), see protocol on back page. Dephosphorylation of proteins (3). Source Arctic shrimp Pandalus borealis. Definition of Activity Unit One unit of the enzyme hydrolyzes 1 µmol of 4-nitrophenylphosphate in 1 min at 37°C. Activity Assay Enzyme activity is assayed in the following mixture: 1 M diethanolamine-HCl (pH 9.8), 0.5 mM MgCl 2 and 10 mM 4-nitrophenylphosphate. Rev.6 V
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Storage Buffer The enzyme is supplied in: 25 mM Tris-HCl (pH 7.6 at 4°C), 1 mM MgCl 2 , 0.1 mM ZnCl 2 and 50% (v/v) glycerol. 10X Reaction Buffer 0.1 M Tris-HCl (pH 7.5 at 37°C), 0.1 M MgCl 2 and 1 mg/ml BSA. Inhibition and Inactivation
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coa_ef0511 - Description CERTIFICATE OF ANALYSIS Shrimp...

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