MIT7_13f08_lab03_Protocol_Biofilm

MIT7_13f08_lab03_Pro - MIT OpenCourseWare http/ocw.mit.edu 7.13 Experimental Microbial Genetics Fall 2008 For information about citing these

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MIT OpenCourseWare http://ocw.mit.edu 7.13 Experimental Microbial Genetics Fall 2008 For information about citing these materials or our Terms of Use, visit: http://ocw.mit.edu/terms .
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7.13 Fall 2008 Page | 1 BIOFILM ASSAY Materials - o/n liquid culture - medium + antibiotic - 1% crystal violet solution - 95% ethanol Method 1) Grow overnight cultures of strains to be assayed. 2) Add 150ul of medium (+ antibiotic if necessary) to a 96-well U-bottom Falcon plate (Falcon #3911). Inoculate 3ul o/n culture into each well. NOTE: Leave several wells of medium un-inoculated. These will serve as controls and should be treated exactly as the inoculated wells in the steps below. 3) Incubate on a rotating platform for 6-8 hours. 4) Discard supernatant and wash with water. This can be done in one the following ways: a. Submerge plate in tub of water. This will simultaneously discard the medium and wash the wells. b.
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This note was uploaded on 11/11/2011 for the course BIO 7.012 taught by Professor Lander during the Fall '10 term at MIT.

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MIT7_13f08_lab03_Pro - MIT OpenCourseWare http/ocw.mit.edu 7.13 Experimental Microbial Genetics Fall 2008 For information about citing these

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