MIT7_13f08_lab05_Protocol_CAS

MIT7_13f08_lab05_Protocol_CAS - Dissolve, while stirring...

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MIT OpenCourseWare http://ocw.mit.edu 7.13 Experimental Microbial Genetics Fall 2008 For information about citing these materials or our Terms of Use, visit: http://ocw.mit.edu/terms .
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CAS Agar for Pseudomonas Note: There is no Mg(II) in the medium 1. Medium, in a 1 l flask 540 ml H 2 O 1.62 g sodium succinate 1.68 g casamino acids 0.43 g Na 2 SO 4 5.44 g PIPES Dissolve, adjust to pH=6.7 (exactly!) with 5 M NaOH 9 g agar Autoclave (with the magnetic stirrer bar still in the flask) 2. CAS solution, in a 100 ml flask 30 ml H 2 O 35 mg chrome azurol S
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Unformatted text preview: Dissolve, while stirring add 6.2 ml 1 mM FeCl 3 (made in 10 mM HCl) 3. Cetrimide-CAS solution 24 ml H 2 O 47 mg cetrimide Dissolve, then add the CAS solution slowly while stirring. Autoclave 4. CAS plates Cool medium to 50C Add Cetrimide-CAS solution slowly while stirring. Pour for low iron CAS plates. Add 1/1000 vol. of 100 mM FeCl 3 (made in 10 mM HCl) to a final conc. of 100 M for high iron CAS plates. UO/2/2001 Expected result for Pseudomonas aeruginosa strain PAO1 and fur mutants on high and low CAS medium plates...
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This note was uploaded on 11/11/2011 for the course BIO 7.012 taught by Professor Lander during the Fall '10 term at MIT.

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MIT7_13f08_lab05_Protocol_CAS - Dissolve, while stirring...

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