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Unformatted text preview: Dissolve, while stirring add 6.2 ml 1 mM FeCl 3 (made in 10 mM HCl) 3. Cetrimide-CAS solution 24 ml H 2 O 47 mg cetrimide Dissolve, then add the CAS solution slowly while stirring. Autoclave 4. CAS plates Cool medium to 50C Add Cetrimide-CAS solution slowly while stirring. Pour for low iron CAS plates. Add 1/1000 vol. of 100 mM FeCl 3 (made in 10 mM HCl) to a final conc. of 100 M for high iron CAS plates. UO/2/2001 Expected result for Pseudomonas aeruginosa strain PAO1 and fur mutants on high and low CAS medium plates...
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This note was uploaded on 11/11/2011 for the course BIO 7.012 taught by Professor Lander during the Fall '10 term at MIT.
- Fall '10