MIT7_13f08_lab17_Protocol_QiagenMiniprep

MIT7_13f08_lab17_Protocol_QiagenMiniprep - MIT...

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MIT OpenCourseWare http://ocw.mit.edu 7.13 Experimental Microbial Genetics Fall 2008 For information about citing these materials or our Terms of Use, visit: http://ocw.mit.edu/terms .
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7.13 Fall 2008 Page | 1 Plasmid DNA Purification Using the QIAprep Spin Miniprep Kit and a Microcentrifuge Before you start Add the provided RNase A solution to Buffer P1, mix, and store at 2–8°C (check for one in the fridge first). Add ethanol (96–100%) to Buffer PE (check label on cap to see if it has been added) before use (see bottle label for volume). Check Buffers P2 and N3 before use for salt precipitation. Redissolve any precipitate by warming to 37°C. Do not shake Buffer P2 vigorously. Close the bottle containing Buffer P2 immediately after use to avoid acidification of Buffer P2 from CO2 in the air. Buffers P2, N3, and PB contain irritants. Wear gloves when handling these buffers All protocol steps should be carried out at room temperature. All centrifugations from step 4 to end in the miniprep protocol are carried out at 13,000
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MIT7_13f08_lab17_Protocol_QiagenMiniprep - MIT...

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