MIT7_13f08_lab18_Protocol_QIAquickGel

MIT7_13f08_lab18_Protocol_QIAquickGel - MIT OpenCourseWare...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
MIT OpenCourseWare http://ocw.mit.edu 7.13 Experimental Microbial Genetics Fall 2008 For information about citing these materials or our Terms of Use, visit: http://ocw.mit.edu/terms .
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
7.13 Fall 2008 Page | 1 QIAquick Gel Extraction Kit Protocol This protocol is designed to extract and purify DNA of 70 bp to 10 kb from standard or low-melt agarose gels in TAE or TBE buffer. Up to 400 mg agarose can be processed per spin column. Read through the QIAquick® Spin Handbook posted at the 7.13 Stellar website for in depth information about this protocol. Notes: • The yellow color of Buffer QG indicates a pH 7.5. • Add ethanol (96–100%, check cap to see if it has already been added) to Buffer PE before use (see bottle label for volume). • Isopropanol (100%) and a heating block at 50°C are required. • All centrifugation steps are carried out at 13,000 rpm (~17,900 x g ) in a conventional table-top microcentrifuge. • 3 M sodium acetate, pH 5.0, may be necessary. 1. Excise the DNA fragment from the agarose gel with a clean, razor. Minimize the size of the gel slice by removing extra agarose.
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 3

MIT7_13f08_lab18_Protocol_QIAquickGel - MIT OpenCourseWare...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online