MIT7_13f08_lab18_Protocol_QIAquickGel - MIT OpenCourseWare...

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MIT OpenCourseWare 7.13 Experimental Microbial Genetics Fall 2008 For information about citing these materials or our Terms of Use, visit: .
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7.13 Fall 2008 Page | 1 QIAquick Gel Extraction Kit Protocol This protocol is designed to extract and purify DNA of 70 bp to 10 kb from standard or low-melt agarose gels in TAE or TBE buffer. Up to 400 mg agarose can be processed per spin column. Read through the QIAquick® Spin Handbook posted at the 7.13 Stellar website for in depth information about this protocol. Notes: • The yellow color of Buffer QG indicates a pH 7.5. • Add ethanol (96–100%, check cap to see if it has already been added) to Buffer PE before use (see bottle label for volume). • Isopropanol (100%) and a heating block at 50°C are required. • All centrifugation steps are carried out at 13,000 rpm (~17,900 x g ) in a conventional table-top microcentrifuge. • 3 M sodium acetate, pH 5.0, may be necessary. 1. Excise the DNA fragment from the agarose gel with a clean, razor. Minimize the size of the gel slice by removing extra agarose.
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MIT7_13f08_lab18_Protocol_QIAquickGel - MIT OpenCourseWare...

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