ps6s05 - MIT Department of Biology 7.013 Introductory...

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1 NAME_______________________________________________TA________ 7.013 Spring 2005 Problem Set 6 FRIDAY April 29th, 2005 Problem sets will NOT be accepted late. Question 1 You are given the following cancer cell line derived from a patient’s cervical tumor. Your job to characterize what caused the tumor in this cell line. You compare this cell line to a cell line derived from the same patient’s normal cervical cells. Normal Cervical Cell a) First you expose the two cell lines to DNA damaging UV radiation and look at the effects. You find that normal cervical cells die after UV exposure, but the cancer cells do not. Which of the following are suggested by this experiment? Circle all that apply. i) a tumor suppressor gene is mutated in this tumor. ii) an oncogene is mutated in this tumor. iii) a gene involved in apoptosis is mutated in this tumor. iv) a gene involved in DNA repair protein is mutated in this tumor . v) a gene involved in metastasis is mutated in this tumor. vi) a gene involved in angiogenesis is mutated in this tumor. b) You look at the location of commonly known cancer genes in the two cell lines by analyzing genetic markers and you determine that there is a loss of heterozygosity on chromosome 13 only in the cancer cells. Using a virus you introduce a wild-type copy of a gene from chromosome 13 known to be involved in carcinogenesis into the cancer cell line. You find that introduction of this gene copy slows down the rate of cell division in the cancer line. Cervical Cancer cell gene from Chromosome 13 MIT Department of Biology 7.013: Introductory Biology - Spring 2005 Instructors: Professor Hazel Sive, Professor Tyler Jacks, Dr. Claudette Gardel
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2 Which of these hypotheses is suggested by this observation? (Circle all that apply.) Chromosome 13 contains... i) an apoptotic factor that is mutated in the cancer cell line. ii) an oncogene that is mutated in the cancer cell line. iii) a tumor suppressor gene that is mutated in the cancer cell line. iv) a gene coding for a DNA repair protein that is mutated in the cancer cell line. v) a gene required for metastasis. vi) a gene required for angiogenesis. c) A colleague discovered a gene that is mutated in late-stage (aggressive) cervical tumors. He gives you a vector containing a wildtype copy of this gene that you transfect into your original cervical cancer cell line. You observe no differences in growth, cell death or any other properties in standard tissue culture tests. You inject these transfected cells, as well as the original cancer cell line (not transfected) into different groups of mice. You observe... Primary Tumors of similar size develop in both groups of mice. The original cancer cell line kills all the mice within 2 month of transfection and dissection of these mice show tumors throughout body.
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