quiz1s05a

quiz1s05a - MIT Department of Biology 7.013: Introductory...

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MIT Department of Biology 7.013: Introductory Biology - Spring 2005 Instructors: Professor Hazel Sive, Professor Tyler Jacks, Dr. Claudette Gardel 7.013 Quiz 1 Answers Question Value 1 28 2 25 3 27 4 20 100 1
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Question 1 You’ve heard that too much sunbathing can be harmful, so before you splurge on a trip to the Bahamas, you decide to test the effects of prolonged UV exposure on DNA. You compare DNA encoding the Sun E protease from a WT E. coli strain with the corresponding DNA from a UV irradiated culture. The highlighted bases below lie in internal piece of the sun E gene encoding the crucial serine (Ser111) in the Sun E protease active site. WT DNA --------------------------cccatgggccaggcgtcg---------- 3’ --------------------------gggtacccggtccgcagc---------- 5’ PROMOTER Encodes Irradiated DNA Translation --------------------------cccatgggctaggcgtct---------- 3’ --------------------------gggtacccgatccgcaga---------- 5’ PROMOTER Start Codon a) Which of the following depicts the mRNA transcribed from the WT DNA nucleotides above? 5 pts i) 5’ -------------------gcugcggaccggguaccc---------- 3’ ii) 5’ -------------------cccaugggccaggcgucg---------- 3’ iii) 5’ -------------------ggguacccgguccgcagc---------- 3’ iv) 5’ -------------------cgacgccuggcccauggg---------- 3’ v) none of the above - it will be _____________________________ b) Which of the following depicts the mRNA transcribed from the Irradiated DNA nucleotides? 5 pts i) 5’ -------------------ggguacccgauccgcaga---------- 3’ ii) 5’ -------------------ucugcggaucggguaccc---------- 3’ iii) 5’ -------------------cccaugggcuaggcgucu---------- 3’ iv) 5’ -------------------agacgccuagcccauggg---------- 3’ v) none of the above - it will be ______________________________ c) Given that the first 3 nucleotides in the WT mRNA written out in a) are in the correct reading frame, what will the WT peptide sequence derived from this segment look like (in single amino acid code)? Written left to right, in the N’ C’ direction. 4 pts i) GYPVRS ii) RRLAHG iii) AADRVP iv) MGQAS v) PMGQAS vi) None of the above – it will be __________________ 2
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d) Given that the first 3 nucleotides in the mRNA transcribed from the Irradiated DNA written out in b) are in the correct reading frame, what will the peptide sequence derived from this segment look like (in single amino acid code)? Written left to right, in the N’ C’ direction. 4 pts i) RRLAHG ii) GYPIRR iii) MGQAS iv) SADRVP v) PMGQAS vi) None of the above – it will be ____ PMG ______________ e) If this was the only region of the sun E gene affected by the UV rays, did irradiation affect the function of Sun E protease? 4 pts
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This note was uploaded on 11/11/2011 for the course BIO 7.012 taught by Professor Lander during the Fall '10 term at MIT.

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quiz1s05a - MIT Department of Biology 7.013: Introductory...

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