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quiz3s05a - MIT Department of Biology 7.013 Introductory...

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7.013 Quiz 3 Answers Question Value Score 1 16 2 31 3 20 4 23 5 10 100 MIT Department of Biology 7.013: Introductory Biology - Spring 2005 Instructors: Professor Hazel Sive, Professor Tyler Jacks, Dr. Claudette Gardel
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2 Question 1 You are a molecular biologist dedicated to preserving rare species from around the world. You recently started a project to clone the rare blue lobster by somatic cell nuclear transfer. This lobster is the same species as the normal purple lobster but has a genetic mutation that gives it a blue exoskeleton. a) Given that you only have somatic, differentiated cells available, what are the two traits desirable for an appropriate donor cell? Choose from the choices below. The best donor cell is…. 4 points haploid diploid a sperm cell densely packed with mitochondria in G 0 or G 1 phase in S phase multinucleated in G2 phase an egg in M phase b) Of these three cell types, which have the ability to give rise to blue lobsters? Cells from the .... (Circle all that apply.) 3 points retina nervous system stomach c) You find that you are only getting viable clones using the intestinal cells. There is a difference in the success of cloning between these cell lines because the____________ is different. Circle all that could correctly fill in the blank. 6 points intracellular Ca+ level mitochondrial genome blueness histone acetylation actin polymerization intracellular ATP level DNA methylation concordance integrin level chromatin structure d) Of your successful blue lobster clones, most but not all of them have behavioral defects. What is/are the likely reason(s) for this? 3 points i) complete reprogramming to embryonic state after nuclear transfer ii) mitochondrial incompatibility with in the egg iii) improper DNA methylation iv) telomeres could be too short v) intestinal cells do not have the glutamate synthase gene Figures by MIT OCW.
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Name__________________________________ Question 2 a) Put values in the boxes of the Y axis on the graph below that depicts changes in the membrane potential of a neuron. 2 points A C ~60 mV +50 mV B D 0 mV Milliseconds b) Circle all correct corresponding terms that describe the neuron at points A-D depicted on the graph above. 10 points. At Point… The neuron is… (Circle one.) The axon’s has its… (Circle all that apply.) A depolarized hyperpolarized mispolarized at resting potential Resting (Leaky) Na+ channels open Voltage-gated Na+ channels open Resting (Leaky) K+ channels open Voltage gated K+ channels open B depolarized hyperpolarized mispolarized at resting potential Resting (Leaky) Na+ channels open Voltage-gated Na+ channels open Resting (Leaky) K+ channels open Voltage gated K+ channels open C depolarized hyperpolarized mispolarized at resting potential Resting (Leaky) Na+ channels open Voltage-gated Na+ channels open Resting (Leaky) K+ channels open Voltage gated K+ channels open D depolarized hyperpolarized mispolarized at resting potential Resting (Leaky) Na+ channels open Voltage-gated Na+ channels open Resting (Leaky) K+ channels open Voltage gated K+ channels open 3
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