r4642dat

r4642dat - Ribonuclease A Solution from bovine pancreas for...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Ribonuclease A Solution from bovine pancreas for molecular biology Catalog Number R4642 Storage Temperature –20 ° C CAS RN 9001-99-4 EC 3.1.27.5 Synonyms: Ribonuclease I; Pancreatic ribonuclease; Ribonuclease 3 -pyrimidinooligonucleotidohydrolase; RNase A; Endoribonuclease I Product Description A major application for Ribonuclease A (RNase A) is the removal of RNA from preparations of plasmid DNA. In this application, the presence of DNase activity as an impurity is a concern. The boiling-water bath method 1 used to eliminate contaminating DNase activity has proven unreliable. For this reason, Sigma developed a proprietary chromatographic preparation method for elimination of DNase activity. RNase A is an endoribonuclease that attacks at the 3 phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA. 2 RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. 3
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 2

r4642dat - Ribonuclease A Solution from bovine pancreas for...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online