Biology 141 - Week 10 slides - Fall 2011 - GM

Biology 141 - Week 10 slides - Fall 2011 - GM - L. Mclane...

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Unformatted text preview: L. Mclane and A. Escobar Metabolic/Biosynthetic Pathways and Mutation Scoring Plates 1. Score color using SD-complete: R (red) or W (white) 2. Score MAT plates (SD) – MATa and MAT α : – “+” = growth, “-” = no growth – Fill in the MAT column with the correct mating type for each strain (HINT: use the info from your mating scoring) 3. Score CAN plate: – R = resistant (MUTANT, can1 ) – S = sensitive (NORMAL, CAN1 ) 4. Score selective plates: Ade, His, Leu – “+” = growth, “-” = no growth L. Mclane and A. Scoring Plates Unknown # Color Ade CAN His Leu MATa tester MAT α tester Mating type 1 W + R + +-+ a 2 R-S-+-+ a 3 R-R + +-+ a 4 R-R-+ +-α 5 W + S---+ a 6 W + R-+ +-α 7 W-S-+ +-α 8 W + R---+ a 9 R-S-+-+ a 10 R-S--+-α 11 R-S + +-+ a 12 W-S + +-+ a 13 W-S---+ a 14 W-S---+ a Be able to look at a table like this and understand the information! Note that the mating type is that of the MAT tester that had no growth! Why do you think this is? L. Mclane and A. Questions to consider 1) How is the CAN plate information different from the information we get from the - nutrient plates? 3) Why did we grow the mating cells (a or ) on YPD first then on SD plates? 4) Why do we use the SD complete plates to score for red or white color? L. Mclane and A. Canavanine Review • CAN1 encodes for arginine permease – Transports ARG and toxic analog canavanine – Competes with ARG during translation to be incorporated into proteins-->non functional proteins • CAN1--> die on plates containing CAN – Can-Sensitive (CanS) • can1--> can grow on plates containing CAN – Can-Resistant (CanR) • In this context, make sure you understand your CAN plate results! L. Mclane and A. L....
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This note was uploaded on 11/14/2011 for the course BIO 141 taught by Professor Dr.cafferty during the Fall '11 term at Emory.

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Biology 141 - Week 10 slides - Fall 2011 - GM - L. Mclane...

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