Biology 141 - Week 11 slides - Fall 2011 - GM

Biology 141 - Week 11 slides - Fall 2011 - GM - •...

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Unformatted text preview: • Analysis of adenine mutant results • Analysis of UV results • UV data worksheet • Design and carry our your own UV experiment! What are you going to do today Mated to Will these cells Make AMP? Genetic Complementation of auxotrophic markers a x α ade6 ade2 Will NOT grow without ADE supplemented ADE2 ade6 ade2 ADE6 WILL grow without ADE supplemented a α Unknown Ade- strain α α ade1 ade2 ade6 SD-ade Predictions • If the patch mated to ade1 and ade2 tester strains, the unknown is what? – ade6 • If the patch mated to ade2 and ade6 tester strains, the unknown is what? – ade1 • If the patch mated to ade1 and ade6 tester strains, the unknown is what? – ade2 Total unknown results From last week: Unknowns #2, 3, 4, 7, 9, 10, 11, 12, 13, 14 All failed to grow on SD-ade plates Therefore, these 10 patches were some sort of adenine mutants Adenine Complementation Results Strain ade1 ade2 ade6 Mutation 1 + + + NONE 2 +- + ade2 3 +- + ade2 4--- TESTER ( ade2 ) 5 + + + NONE 6...
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This note was uploaded on 11/14/2011 for the course BIO 141 taught by Professor Dr.cafferty during the Fall '11 term at Emory.

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Biology 141 - Week 11 slides - Fall 2011 - GM - •...

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