8-metabolism-2[1]

8-metabolism-2[1] - Note: Ive included more slides here...

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Note: I’ve included more slides here than I showed in lecture; be sure to take a look at the ones I didn’t show, as you will want to know that information as well (per my discussion board post about the cancelled lecture).
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Welcome back! Today we’ll continue with metabolism And talk a bit about literature citation A rough draft of your first journal article is due Wednesday/Thursday in lab Along with your group’s experimental proposal The lab exam is today/tomorrow You must take the exam in your registered section The lab exam will start at the beginning of lab
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Metabolism unit outline 1. Energy / carbon source distinctions 2. Eukaryote metabolism Oxidative respiration Fermentation 3. Bacterial metabolism – overview 4. Oxidation / reduction – the electron tower 5. Bacterial metabolism detail 1. Chemolithotropes 2. Chemoorganotrophs 3. Fermentation 6. How do bacteria get carbon?
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Bacteria do exactly the same thing … Except they have a much wider range of the molecules they use as electron donors and acceptors Organic compounds O 2 CO 2 boring Eukaryotes
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To make sense of bacteria … We need to look at oxidation/reduction a little more E 0 - The reduction potential of a molecule A number showing the tendency for a molecule to become oxidized or reduced Always expressed for half reactions that are written as reductions The more negative, the stronger of a reductant the pair is e.g. 2H + + 2e - H 2 ; E 0 = -0.42 The more positive, the stronger of an oxidant the pair is e.g. ½O 2 + 2H + + 2e - H 2 O; E 0 = +0.82 E 0 values are at pH 7
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Electron tower Organized by E 0 Strong reductants are on top (glucose) Strong oxidants are on the bottom (O 2 ) The amount of energy liberated by a reaction ( Δ G 0 ) is determined by the change in reduction potential ( Δ E 0 ) Δ G 0 α Δ E 0
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Electron tower Δ E 0 = -0.45 Δ E 0 = -0.84 Δ E 0 = -1.24
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Electron tower Δ E 0 = -0.45 Δ E 0 = -0.84 Δ E 0 = -1.24
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Electron tower Δ E 0 = -0.45 Δ E 0 = -0.84 Δ E 0 = -1.24
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Which of these pairs would net the most ATP? 1. Methanol + Fe 3+ (ph 7) 2. ½N 2 + ½ O 2 3. Glucose + Pyruvate 4. H 2 S + NO 3 - -> NO 2 - + S 0
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Which of these pairs would net the most ATP? 1. Methanol + Fe 3+ (ph 7) 2. ½N 2 + ½ O 2 3. Glucose + Pyruvate 4. H 2 S + NO 3 - -> NO 2 - + S 0
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Which of these pairs would be a chemolithotrope? 1. Lactate + Fe 3+ (ph 7) 2. ½N 2 + ½ O 2 3. Glucose + Pyruvate 4. H 2 S + NO 2 - 5. 6. 7. 8. 9. All of the above
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Chemoorganotrophic bacteria Can be either Aerobic – using O 2 as the terminal electron acceptor Anaerobic – using something other than O 2 as the terminal electron acceptor Get less energy out of molecules, since O 2 is the best biological oxidant Most generate ATP using a proton motive force (just like Euk’s) S 0 = sulfur; NO 3 - = nitrate; SO 4 2- = sulfate
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chemolithotropes
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8-metabolism-2[1] - Note: Ive included more slides here...

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