lab report#2 - The Reaction Rate of an Enzyme Based on...

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The Reaction Rate of an Enzyme Based on Concentration and pH Jonathan Grant Andrew Z. Introduction Enzymes are protein molecules that act as catalysts for chemical reactions within living cells. They reduce the amount of energy needed to start the reaction. There are different types of reactions, but a particular enzyme is usually specific to only one particular reaction. Regardless of the type, all enzymatic reactions share similar properties. When the enzyme binds the substrate molecule, this is called the active site. This result usually only fits one substrate. This explains substrate specificity. The shape and active site of an enzyme are very sensitive to temperature and pH changes. Denaturation is when the normal shape of the enzyme is lost. The enzyme then loses the ability to catalyze reactions. A narrow range of temperature and pH is best for enzymes.
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The reactant, or substrate molecule, combines with the enzyme to catalyze a reaction. This forms an enzyme-substrate complex. During this reaction, the enzyme molecule is reused again and again. A single enzyme molecule may catalyze the conversion of millions of the appropriate substrate molecules to product molecules. Inhibitors slow the rate of an enzymatic reaction. These are compounds that permanently bond with the active site and prevent the substrate molecule from joining with the enzyme. They may also just change the shape of the enzyme and its active site. In the lab, we examined how the rate of an enzyme reaction is affected by different enzyme concentrations, different substrates, and changes in environmental pH. The enzyme studied was catechol oxidase. The general characteristics of enzymes as biological catalysts were understood by the end of the lab. We had an objective to see how a colorimeter works and how absorbance is used to measure product formation. We understood how to determine the relationship between slope and reaction rate. We hypothesized a few things about the lab. With enzyme concentration, the cuvette with the most drops of enzyme would have the fastest reaction
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lab report#2 - The Reaction Rate of an Enzyme Based on...

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