AP_Bio_-_Spectrophotometry

AP_Bio_-_Spectrophotometry - Applications of...

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Spectrophotometry
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Does a spectrophotomer do? Detects how much light passes through a sample Who cares about this? Gives you insight into what is in your sample
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How does this work?
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Vary the wavelength (keep conc of sample the same) Determine the “absorption spectrum” Like a molecular fingerprint – λ max is easy to spot – place where absorbance is the highest % Transmittance can be converted to absorption
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Vary the concentration (keep wavelength the same) Absorption is directly proportional to concentration
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Beer’s law relates absorption to concentraiton A = ε c l ε – molar absorptivity c – concentration of sample l – path length for sample
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Unformatted text preview: Applications of spectrophotometry are varied: Some examples Determine the absorption spectrum for a newly discovered molecule Food & drug analysis WHAT is in a sample Measuring concentration of a sample in solution Measuring how the concentration of a sample changes over time Ex measure change in concentration of a substrate or product to determine reaction rate Consider. . What limitations does this have for us when studying an enzyme mediated reaction? Substance must Be Colored Be Dissolved in solution Have a max that does not coincide with other molecules in the solution...
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This note was uploaded on 11/16/2011 for the course BIO 101 taught by Professor Martin during the Fall '08 term at Rutgers.

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AP_Bio_-_Spectrophotometry - Applications of...

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