Lab report NUMBER 1

Lab report NUMBER 1 - Ben Jasper The Effects of PH and...

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Ben Jasper The Effects of PH and Temperature on alkaline phosphate I. Abstract- In this lab Enzymes were used to illustrate that they could catalyze reactions or speed up the rate of products. Enzymes were tested in various different environments but our lab focused on two in particular. They consisted of how pH effects enzyme reactions and how does temperature effect enzyme reactions. We were able to measure the total production of pNp (product) by using the spec 20. We were able to measure how acidic each reaction was by placing a sample of each enzyme reaction on a piece of P.H. paper. In conclusion our group found out that this particular enzyme, “Alkaline Phosphatase” is most effective at mild temperatures but especially warm temperatures around 45 degree’s Celsius . As the temperature increased in each enzyme reaction, so did the rate of production. It also is very high product formed at a pH buffer solution of about ten and a very low rate of production at a pH of 4, which indicated that it thrives in basal solutions not acidic ones. Overall, one could conclude that as the enzyme reactions became more basal, the enzyme activity increased, and as the temperature increased the rate of production of pNp increased. II. Introduction- The reason this lab was conducted was for students to obtain a better understanding of how enzyme reactions work by doing several experiments. In this lab students worked with enzymes and substrates and
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found precise measurements of product. Enzymes are catalysts that speed up the reaction of products formed by lowering the amount of activation needed . Substrates are the reactants in which enzymes temporarily bind to (Davis, martin, and Associates). Product is the result of a given reaction that is measurable. In this lab students measured the rate of color formed over a given period of time by using the Spec 20. In Exercise III of the lab students focused their attention on the effect of pH and temperature and how they affected enzyme catalyzed reactions. In Assay one or the assay that tests effects of pH buffers on enzyme reactions one could predict that enzymes in acidic pHs tend not to catalyze at all but when you place a enzyme in a basal solution enzyme activity tends to thrive (Solomon, E.L.) . My assumption for why Alkaline Phosphastase was more affective in basal solutions is because enzymes thrive in basal solutions and not in acidic ones, thus creating more product in basal solutions and we went over that information in bio lecture. In assay two the variable that was being measured was temperature and this was orchestrated in a study in which one group was a control group (which contained no enzyme) and another was an experimental group. (Which contained the enzyme) The control group consisted of just a pH buffer of 8 and substrate, while the experimental had both of these as well and an enzyme added to the mix. (Alkaline phosphatase) Given that enzymes are supposed to speed up reactions and they require
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This note was uploaded on 11/16/2011 for the course BIO 101 taught by Professor Martin during the Fall '08 term at Rutgers.

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Lab report NUMBER 1 - Ben Jasper The Effects of PH and...

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