Lecture 24 SRR F-2011

Lecture 24 SRR F-2011 - Biology 313 Lecture 24 0 ISU Life...

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Biol 394 in Spain (3 or 6 credits): ISU on the Mediterranean http://www.language.iastate.edu/valencia/ Biology 313, Lecture 24 Oct 21, 20110 ISU Life Science students at the biggest comic book store in Spain (Madrid)
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Chapter 10 : DNA– Chemical Nature of the Gene Problems (through Section 10.3) Concept Checks: 1-8 Worked Problems: 1 Comprehension and Application Questions at end of Chapter: 1-14, 17-21, 24, 25, 27, 28, 31-34 (19, 21, 22, 23 answers in “Assignments” on Blackboard)
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Erwin Chargaff (1940’s, Columbia, NYC): measured amounts of the various bases in DNAs from a variety of organisms Chargaff’s Rules: The amount of T = amount of A (A/T = 1.0) The amount of C = amount of G (C/G = 1.0) Thus, (A + G) / (T + C) = 1.0 Early studies of DNA: what is it?
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Conclusions: 1. Most species are AT-rich Chargaff’s Rules 2. Base compositions vary from species-to- species
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Chargaff’s Rules (VERY IMPORTANT) Because base compositions vary from species-to-species, the tetranucleotide theory is incorrect (there IS variability in DNA) Chargaff’s Rules were the clue Watson and Crick needed to solve the structure of DNA
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Chapter 10 : DNA– Chemical Nature of the Gene 1. Characteristics of genetic material 2. Early studies of DNA: what is it? 3. Early evidence that DNA is the genetic material A. The “transforming principle” B. Hershey-Chase 4. RNA as the genetic material 5. Structure of DNA
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Two early, landmark experiments showed that DNA is the genetic material DNA is the “transforming principle” in the bacterium Streptococcus pneumoniae (causes pneumonia): Griffith (1928); Avery, MacLeod & McCarty (1944) (To Elmo)
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Lecture 24 SRR F-2011 - Biology 313 Lecture 24 0 ISU Life...

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